7F6L

Crystal structure of human MUS81-EME2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-12-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 41
Unit cell lengths	93.097, 93.097, 87.993
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.440 - 3.200
Rwork	0.229
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p0p
RMSD bond length	0.011
RMSD bond angle	1.640
Data scaling software	HKL-3000 (1.13_2998)
Phasing software	MOLREP (1.13_2998)
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.440	3.260
High resolution limit [Å]	3.199	3.200
Number of reflections	12507	1236
<I/σ(I)>	16.63
Completeness [%]	99.7
Redundancy	13.8
CC(1/2)	0.997	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	8% v/v TacsimateTM pH 7.0 and 20% w/v Polyethylene glycol 3,350