7F4E

Crystal structure of Hst2 in complex with H3K9bz peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97855
Spacegroup name	P 21 21 21
Unit cell lengths	42.150, 68.402, 94.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.610 - 1.780
R-factor	0.1853
Rwork	0.184
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q1a
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.810
High resolution limit [Å]	1.780	4.830	1.780
Rmerge	0.063	0.042	0.802
Rmeas	0.069	0.046	0.901
Rpim	0.027	0.019	0.399
Total number of observations	167194
Number of reflections	26704	1483	1203
<I/σ(I)>	6.7
Completeness [%]	98.9	98.8	89.9
Redundancy	6.3	6.1	4.5
CC(1/2)		0.997	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	25% (w/v) PEG 1500, 0.1M MMT/Sodium hydroxide