7F2X
Crystal structure of MEK1 C121S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 49.498, 156.504, 179.156 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.789 - 2.007 |
| R-factor | 0.2159 |
| Rwork | 0.215 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eqc |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.583 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.789 | 2.130 |
| High resolution limit [Å] | 2.007 | 2.007 |
| Rmerge | 0.198 | 1.207 |
| Number of reflections | 23080 | 3547 |
| <I/σ(I)> | 5.59 | 1.15 |
| Completeness [%] | 97.8 | 95 |
| Redundancy | 6.8 | 6.6 |
| CC(1/2) | 0.973 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | PEG 3350 26%, 100 mM Bicine, 150 mM NH4F, 1% glycerol |
