7F2F
The complex of DNA with the C-terminal domain of TYE7 from Saccharomyces cerevisiae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.025, 73.025, 181.782 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.760 - 2.550 |
| R-factor | 0.2478 |
| Rwork | 0.246 |
| R-free | 0.28380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1am9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.884 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.760 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 16802 | 818 |
| <I/σ(I)> | 33.15 | 2.05 |
| Completeness [%] | 99.9 | |
| Redundancy | 17.9 | |
| CC(1/2) | 0.995 | 0.876 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.1M HEPES pH7.5, 10% w/v PEG 8000, 11% v/v ethylene glycol |
