7F1E
Structure of METTL6 bound with SAM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 43 |
Unit cell lengths | 83.457, 83.457, 129.686 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.857 - 2.589 |
R-factor | 0.1934 |
Rwork | 0.191 |
R-free | 0.22650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nec |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.857 | 2.630 |
High resolution limit [Å] | 2.589 | 2.589 |
Rmerge | 0.094 | 1.056 |
Number of reflections | 41739 | 870 |
<I/σ(I)> | 20.8 | |
Completeness [%] | 99.9 | |
Redundancy | 12.5 | |
CC(1/2) | 0.992 | 0.699 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Sodium acetate pH5.2, 2.0M sodium formate |