7F0S
A crystal structure of alphavirus nonstructural protein 4 (nsP4) reveals an intrinsically 1dynamic RNA-dependent RNA polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.837, 68.349, 99.646 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.130 - 2.600 |
R-factor | 0.2664 |
Rwork | 0.265 |
R-free | 0.28840 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.976 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.064 | 4.800 |
Number of reflections | 37774 | |
<I/σ(I)> | 12.04 | 0.36 |
Completeness [%] | 99.8 | 98.4 |
Redundancy | 5.69 | 5.69 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES/TRIS, 24% PEG 3350, 0.2M Ammonium Acetate, 0.3% TMAO |