7F0F
Crystal structure of capreomycin phosphotransferase in complex with CMN IIB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2020-12-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9732 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 92.101, 92.101, 119.006 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.150 - 2.100 |
| R-factor | 0.1891 |
| Rwork | 0.186 |
| R-free | 0.22280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f0a |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.926 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 30103 | 2918 |
| <I/σ(I)> | 49.02 | 3.45 |
| Completeness [%] | 98.5 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.976 | 0.897 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.4 M sodium malonate pH 7.0, 0.1 M Bis-Tris propane pH 7.0, 0.1 M calcium chloride dihydrate |
