7EZT

The structure and functional mechanism of nucleotide regulated acetylhexosaminidase Am2136 from Akkermansia muciniphila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-06-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97930
Spacegroup name	P 1 21 1
Unit cell lengths	96.200, 119.505, 161.931
Unit cell angles	90.00, 103.40, 90.00

Refinement procedure

Resolution	26.440 - 2.810
R-factor	0.195
Rwork	0.194
R-free	0.24790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jqf
RMSD bond length	0.009
RMSD bond angle	1.352
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.020
High resolution limit [Å]	2.810	2.810
Rpim	0.065	0.244
Number of reflections	86269	8562
<I/σ(I)>	11.6	3
Completeness [%]	99.2
Redundancy	13.7
CC(1/2)	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.2M Li2SO4, 25% w/v PEG3350, 0.1M HEPES, pH7.5