7EWH
Crystal structure of human PHGDH in complex with Homoharringtonine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-01-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.165, 120.639, 59.480 |
| Unit cell angles | 90.00, 100.72, 90.00 |
Refinement procedure
| Resolution | 27.555 - 2.990 |
| R-factor | 0.2479 |
| Rwork | 0.237 |
| R-free | 0.34800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g76 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.555 | 3.097 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.136 | |
| Rmeas | 0.529 | |
| Rpim | 0.281 | |
| Number of reflections | 9221 | 481 |
| <I/σ(I)> | 9.002 | |
| Completeness [%] | 76.0 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 mM MMT buffer, pH 7.0 with 30% PEG4000 |
