7EVU
Co-crystal Structure of Toxoplasma gondii Prolyl tRNA Synthetase (TgPRS) in complex with HFG and JE6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2020-07-17 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.754, 70.830, 76.036 |
| Unit cell angles | 92.16, 101.44, 115.61 |
Refinement procedure
| Resolution | 54.240 - 1.697 |
| R-factor | 0.1964 |
| Rwork | 0.195 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6aa0 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15rc2_3428) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.970 | 1.730 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 138636 | 6454 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 96.9 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.989 | 0.314 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Morpheus G2 |
