7EPM
human LDHC complexed with NAD+ and ethylamino acetic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 177.891, 177.891, 179.210 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.470 - 3.000 |
R-factor | 0.2044 |
Rwork | 0.203 |
R-free | 0.22650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i10 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.188 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.070 |
High resolution limit [Å] | 2.998 | 2.998 |
Number of reflections | 34121 | 2229 |
<I/σ(I)> | 15 | |
Completeness [%] | 100.0 | |
Redundancy | 21.3 | |
CC(1/2) | 0.987 | 0.602 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M sodium chloride, 0.1 M HEPES pH 7.5, 1.6 M ammonium sulfate |