7EOV
Crystal structure of mouse cytosolic sulfotransferase mSULT2A8 in complex with PAP and cholic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2007-04-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 94.564, 94.564, 244.555 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.400 - 2.600 |
R-factor | 0.2359 |
Rwork | 0.234 |
R-free | 0.26520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q22 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.036 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.300 | 2.730 |
High resolution limit [Å] | 2.600 | 2.600 |
Rpim | 0.022 | 0.549 |
Number of reflections | 17560 | 2425 |
<I/σ(I)> | 24.4 | 1.5 |
Completeness [%] | 99.6 | |
Redundancy | 14.3 | |
CC(1/2) | 1.000 | 0.741 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.5M ammonium sulfate, 0.1M tri-sodium citrate dihydrate pH 5.6, 0.7M lithium sulfate |