7EOD
MITF HLHLZ Delta AKE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97852 |
Spacegroup name | P 1 |
Unit cell lengths | 33.020, 52.880, 55.470 |
Unit cell angles | 82.64, 85.49, 71.85 |
Refinement procedure
Resolution | 39.160 - 1.900 |
R-factor | 0.1907 |
Rwork | 0.188 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7d8r |
Data reduction software | DIALS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 1.940 |
High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
Rmerge | 0.074 | 0.021 | 0.684 |
Rmeas | 0.087 | 0.024 | 0.804 |
Rpim | 0.045 | 0.012 | 0.419 |
Number of reflections | 49812 | 235 | 1705 |
<I/σ(I)> | 11.7 | ||
Completeness [%] | 93.9 | 92.9 | 93.7 |
Redundancy | 3.6 | 3.6 | 3.5 |
CC(1/2) | 0.998 | 0.999 | 0.817 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.8 M Ammonium citrate pH 7.0 |