7EMC
Mooring Stone-Like Arg114 Pulls Diverse Bulged Peptides: First Insight into African Swine Fever Virus-Derived T Cell Epitopes Presented by Swine Major Histocompatibility Complex Class I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.534, 110.632, 136.228 |
Unit cell angles | 90.00, 95.42, 90.00 |
Refinement procedure
Resolution | 45.210 - 1.900 |
R-factor | 0.1825 |
Rwork | 0.180 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qq3 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.836 |
Data scaling software | XDS (1.13_2998) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.077 | 0.219 |
Number of reflections | 95001 | 95001 |
<I/σ(I)> | 8.92 | |
Completeness [%] | 98.3 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1M BIS-TRIS pH6.5,16%w/v polyethylene glycol 10000 |