7EM7

Crystal structure of the PI5P4Kbeta N203D-XTP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	95
Detector technology	PIXEL
Collection date	2020-09-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	109.213, 185.857, 106.961
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.640 - 3.450
R-factor	0.2416
Rwork	0.239
R-free	0.29620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7C55
RMSD bond length	0.011
RMSD bond angle	1.293
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.640	3.640
High resolution limit [Å]	3.450	3.450
Rmerge	0.093	0.891
Number of reflections	14659	2134
<I/σ(I)>	15.08
Completeness [%]	99.8
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	9%(W/V) PEG4000, 0.1 M Sodium Citrate pH 6.0, 0.1 M Magnesium Acetate, 0.1 M Lithium Acetate