7EM4
Crystal structure of the PI5P4Kbeta F205L-ITP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2018-12-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 109.311, 185.530, 105.744 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.935 - 2.800 |
R-factor | 0.2193 |
Rwork | 0.216 |
R-free | 0.27750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6k4h |
RMSD bond length | 0.009 |
RMSD bond angle | 1.096 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.550 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.062 | 0.890 |
Number of reflections | 26836 | 3817 |
<I/σ(I)> | 28.86 | 2.7 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 8.9 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 9%(w/v) PEG4000, 0.1 M sodium citrate pH 6.0, 0.1 M magnesium acetate, 0.1 M lithium acetate |