7EM2
Crystal structure of the PI5P4Kbeta-XTP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2018-11-22 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 109.848, 185.138, 105.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.103 - 2.600 |
R-factor | 0.2215 |
Rwork | 0.220 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3x04 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.470 | 2.740 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.034 | 0.929 |
Number of reflections | 33540 | 4827 |
<I/σ(I)> | 39.97 | 2.88 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.7 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 9%(w/v) PEG4000, 0.1 M sodium citrate pH 6.0, 0.1 M magnesium acetate, 0.1 M lithium acetate |