7EL4

The crystal structure of p53p peptide fragment in complex with the N-terminal domain of MdmX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	I 41
Unit cell lengths	65.097, 65.097, 94.977
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.700 - 2.110
Rwork	0.224
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c3y
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (REFMAC5)

Data quality characteristics

	Overall
Low resolution limit [Å]	53.695
High resolution limit [Å]	2.109
Number of reflections	22483
<I/σ(I)>	17
Completeness [%]	100.0
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.16	291	0.1 M MES pH 6.5, 2 M MgSO4