7EIK

RD4-BD1 in complex with LT-872-297

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	31.820, 47.340, 82.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.060 - 1.700
R-factor	0.2313
Rwork	0.228
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uy9
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.060	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.113	0.956
Number of reflections	10914	579
<I/σ(I)>	4.6
Completeness [%]	77.7
Redundancy	2.7
CC(1/2)	0.986	0.376

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	4 M sodium formate