7EIC
Crystal structure of AF9 YEATS domain in complex with H4K5acK8ac peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-16 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 30.367, 51.702, 56.273 |
Unit cell angles | 89.30, 74.81, 89.87 |
Refinement procedure
Resolution | 50.010 - 1.950 |
R-factor | 0.17823 |
Rwork | 0.177 |
R-free | 0.20475 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tmp |
RMSD bond length | 0.005 |
RMSD bond angle | 1.137 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 22633 | 965 |
<I/σ(I)> | 13.9 | 3.1 |
Completeness [%] | 94.1 | |
Redundancy | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Bis-Tris buffer (pH 6.5) containing 100 mM ammonium acetate and 25% PEG3350 |