7EHE

Acetolactate Synthase from Trichoderma harzianum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97855
Spacegroup name	P 21 21 21
Unit cell lengths	79.100, 88.530, 188.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.260 - 2.280
R-factor	0.1977
Rwork	0.196
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dek
RMSD bond length	0.008
RMSD bond angle	0.968
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.030	2.340
High resolution limit [Å]	2.280	2.280
Number of reflections	61042	4441
<I/σ(I)>	15.4
Completeness [%]	100.0
Redundancy	12.8
CC(1/2)	0.999	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	18% PEG4000 0.2 M CaCl2 0.1 M Tris 8.5