7EDP

Crystal structure of AF10-DOT1L complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9788
Spacegroup name	P 31 2 1
Unit cell lengths	37.524, 37.524, 157.445
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.826 - 2.200
R-factor	0.2261
Rwork	0.221
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zik
RMSD bond length	0.007
RMSD bond angle	0.866
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (dev_1951)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.826	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.124
Rmeas	0.132
Rpim	0.043
Number of reflections	6826	237
<I/σ(I)>	22.7
Completeness [%]	95.5
Redundancy	8.9
CC(1/2)		0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% Ethylene glycol