7ECT
Crystal Structure of Aspergillus terreus Glutamate Dehydrogenase (AtGDH) Complexed With Tartrate and NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-08-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 119.860, 153.090, 259.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.290 - 2.900 |
| R-factor | 0.22491 |
| Rwork | 0.223 |
| R-free | 0.26853 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xvx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.587 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.290 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.420 | 1.520 |
| Rmeas | 0.460 | 1.650 |
| Number of reflections | 53084 | 5074 |
| <I/σ(I)> | 4.73 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.7 | 6.7 |
| CC(1/2) | 0.960 | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 295 | 0.2 M Sodium tartrate dibasic, 20% PEG3350 pH 7.3 |
