7ECR
Crystal Structure of Aspergillus terreus Glutamate Dehydrogenase (AtGDH) Complexed With Succinate and ADP-ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-07-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 119.880, 153.750, 259.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.680 - 1.730 |
| R-factor | 0.14616 |
| Rwork | 0.145 |
| R-free | 0.16700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xvx |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.120 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.680 | 1.830 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.110 | 0.910 |
| Rmeas | 0.110 | 0.100 |
| Number of reflections | 246054 | 36141 |
| <I/σ(I)> | 15.38 | 2.1 |
| Completeness [%] | 99.2 | 94.7 |
| Redundancy | 7.11 | 5.93 |
| CC(1/2) | 0.990 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.8 M Succinic acid, pH 7.0 |
