7ECR

Crystal Structure of Aspergillus terreus Glutamate Dehydrogenase (AtGDH) Complexed With Succinate and ADP-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-07-06
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	119.880, 153.750, 259.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.680 - 1.730
R-factor	0.14616
Rwork	0.145
R-free	0.16700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xvx
RMSD bond length	0.023
RMSD bond angle	2.120
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.680	1.830
High resolution limit [Å]	1.730	1.730
Rmerge	0.110	0.910
Rmeas	0.110	0.100
Number of reflections	246054	36141
<I/σ(I)>	15.38	2.1
Completeness [%]	99.2	94.7
Redundancy	7.11	5.93
CC(1/2)	0.990	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.8 M Succinic acid, pH 7.0