7EBC

Crystal structure of Isocitrate lyase-1 from Saccaromyces cervisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	95.522, 129.377, 210.787
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.800 - 2.300
R-factor	0.19046
Rwork	0.189
R-free	0.21974
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e9g
RMSD bond length	0.004
RMSD bond angle	0.640
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	116065	11263
<I/σ(I)>	13.8
Completeness [%]	99.7
Redundancy	6.9
CC(1/2)	0.997	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	40mM Na-HEPES pH7.0, 8%(w/v)PEG 3350