7E9V

The Crystal Structure of human UMP-CMP kinase from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 65 2 2
Unit cell lengths	61.926, 61.926, 225.397
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.473 - 2.100
Rwork	0.205
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tev
RMSD bond length	0.009
RMSD bond angle	1.636
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.473	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.075	0.937
Number of reflections	15960	1267
<I/σ(I)>	24
Completeness [%]	100.0
Redundancy	18.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.5M (NH4)2SO4, 0.1M Tris pH8.5