7E9K
Crystal Structure of POMGNT2 in complex with UDP and mono-mannosyl peptide (379Man long peptide)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.150, 104.401, 148.639 |
Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
Resolution | 49.550 - 2.050 |
R-factor | 0.204 |
Rwork | 0.202 |
R-free | 0.24070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_1300018733 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.543 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.570 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.070 | 0.801 |
Rpim | 0.044 | 0.600 |
Number of reflections | 163077 | 7958 |
<I/σ(I)> | 10.8 | 1.6 |
Completeness [%] | 99.0 | 99 |
Redundancy | 3.5 | |
CC(1/2) | 0.999 | 0.761 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M Tris-HCl (pH 8.0 or 8.5), 12-16% PEG 8000, 1% tacsimate (pH 7.0) |