7E5O
Crystal structure of SARS-CoV-2 RBD in complex with antibody NT-193
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.073, 106.886, 148.074 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.100 - 2.800 |
R-factor | 0.2277 |
Rwork | 0.226 |
R-free | 0.25620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7jmp |
RMSD bond length | 0.007 |
RMSD bond angle | 0.982 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.100 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.205 | |
Rmeas | 0.223 | |
Rpim | 0.085 | |
Number of reflections | 33546 | 3306 |
<I/σ(I)> | 5.75 | |
Completeness [%] | 99.9 | |
Redundancy | 6.8 | |
CC(1/2) | 0.969 | 0.704 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.5, 10% (w/v) PEG 8000, 8% (w/v) Ethylene glycol |