7E5A
interferon-inducible anti-viral protein R356A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 114.260, 114.260, 168.490 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.480 - 2.500 |
R-factor | 0.2131 |
Rwork | 0.211 |
R-free | 0.24990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f5n |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
Rmerge | 0.123 | 0.075 | 1.294 |
Rmeas | 0.131 | 0.081 | 1.376 |
Rpim | 0.041 | 0.028 | 0.455 |
Total number of observations | 401695 | ||
Number of reflections | 44229 | 2310 | 2199 |
<I/σ(I)> | 5.5 | ||
Completeness [%] | 98.7 | 95.5 | 98.6 |
Redundancy | 9.1 | 8.2 | 7.9 |
CC(1/2) | 0.983 | 0.639 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 289.15 | ammonium sulfate, HEPES pH 7.5, polyethylene glycol 3350 |