7E58

interferon-inducible anti-viral protein 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	42.207, 124.161, 355.722
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.030 - 2.600
R-factor	0.2555
Rwork	0.255
R-free	0.27150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f5n
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	120.000	120.000	2.640
High resolution limit [Å]	2.600	7.060	2.600
Rmerge	0.151	0.059	1.325
Rmeas	0.162	0.063	1.421
Rpim	0.057	0.021	0.494
Total number of observations	414059
Number of reflections	55672	3315	2117
<I/σ(I)>	4.3
Completeness [%]	92.4	99.4	70.8
Redundancy	7.4	8.6	7.2
CC(1/2)		0.998	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		289.15	2-propanol, sodium citrate, tribasic dihydrate pH 5.0, polyethylene glycol 10,000