7E55
Cryo-EM structure of alpha 7 homo-tetradecamer
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | JEOL 2200FS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 4.2 |
| Energy Filter | In-column Omega Filter |
| Detector | DIRECT ELECTRON DE-20 (5k x 3k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.9 |
| Software | RELION,CTFFIND,PHENIX |
| Symmetry | D7 |
| CTF correction | PHASE FLIPPING ONLY |
