7E53
Crystal structure of sfGFP complexed with the nanobody nb2 at 2.2 Angstron resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97875 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 64.207, 123.991, 108.876 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 4.730 | 2.200 |
Rmerge | 0.258 | 0.246 | 0.467 |
Rmeas | 0.273 | 0.259 | 0.517 |
Rpim | 0.086 | 0.077 | 0.212 |
Total number of observations | 133097 | ||
Number of reflections | 21347 | 2342 | 1943 |
<I/σ(I)> | 9.8 | ||
Completeness [%] | 96.0 | 99.4 | 89.1 |
Redundancy | 6.2 | 10 | 4.1 |
CC(1/2) | 0.964 | 0.340 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% (w/v) Polyethylene glycol 400, 100mM CAPS/ Sodium hydroxide, pH 10.5 |