7E50

Crystal structure of human microplasmin in complex with kazal-type inhibitor AaTI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	72.895, 86.044, 50.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.780 - 1.950
R-factor	0.2224
Rwork	0.218
R-free	0.27480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d3x
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.720	1.890
High resolution limit [Å]	1.850	1.850
Rpim	0.057	0.602
Number of reflections	28769	1736
<I/σ(I)>	7.8
Completeness [%]	99.6
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.2 M Ammonium formate, 20 % polyethylene glycol 3350 pH 6.6