7E4Z

Crystal structure of tubulin in complex with Maytansinol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97908
Spacegroup name	P 21 21 21
Unit cell lengths	104.431, 156.635, 182.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.285 - 2.690
R-factor	0.194
Rwork	0.192
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tv8
RMSD bond length	0.002
RMSD bond angle	0.646
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.800
High resolution limit [Å]	2.690	5.810	2.700
Rmerge	0.133	0.057	0.723
Rmeas	0.138	0.060	0.750
Rpim	0.037	0.017	0.199
Number of reflections	83413	8729	8212
<I/σ(I)>	4.2
Completeness [%]	100.0	100	100
Redundancy	13.4	12.5	13.9
CC(1/2)		0.998	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2