7E3X
Crystal structure of SDR family NAD(P)-dependent oxidoreductase from exiguobacterium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-12-25 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 68.464, 71.167, 115.158 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.760 - 2.580 |
Rwork | 0.192 |
R-free | 0.25480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5t2u |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.580 | 2.600 |
Number of reflections | 17891 | 821 |
<I/σ(I)> | 1.6 | 1.4 |
Completeness [%] | 97.7 | |
Redundancy | 4.8 | |
CC(1/2) | 0.948 | 0.570 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | PEG 3350 |