7E17

Structure of dimeric uPAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	79.580, 79.580, 270.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.270 - 2.960
R-factor	0.2505
Rwork	0.248
R-free	0.29930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fd6
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.270	56.270	3.040
High resolution limit [Å]	2.960	13.240	2.960
Rmerge	0.175	0.042	1.312
Rmeas	0.191	0.048	1.417
Number of reflections	37874	266	1347
<I/σ(I)>	10.25	21.83	2.05
Completeness [%]	99.1	91.4	99.9
Redundancy	6.74	5.128	7.272
CC(1/2)	0.994	0.996	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	1.8-2.2 M ammonium sulphate in 50 mM sodium acetate at pH 4.5oC5.2