7E0E

Crystal structure of mouse interferon alpha2 at 2.1 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	42.733, 42.733, 180.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.803 - 2.102
R-factor	0.1896
Rwork	0.188
R-free	0.21530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1itf
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.803	29.803	2.230
High resolution limit [Å]	2.100	6.250	2.100
Rmerge	0.079	0.021	0.650
Rmeas	0.083	0.023	0.677
Total number of observations	130066
Number of reflections	10532	481	1654
<I/σ(I)>	26.01	78.63	4.1
Completeness [%]	100.0	99.8	100
Redundancy	12.35	10.052	12.808
CC(1/2)	1.000	1.000	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MES, PEG 20000