7DZM
Crystal Structure of the cross-restricted T18A TCR and HLAB8101 bound to HIV-1 Gag TL9 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2019-12-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 93.098, 93.098, 263.051 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.550 - 2.250 |
R-factor | 0.1974 |
Rwork | 0.196 |
R-free | 0.23990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u1i 4gg6 4udt |
RMSD bond length | 0.006 |
RMSD bond angle | 0.822 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.550 | 2.322 |
High resolution limit [Å] | 2.250 | 2.242 |
Rmerge | 0.034 | 0.373 |
Rmeas | 0.048 | 0.528 |
Number of reflections | 56526 | 5547 |
<I/σ(I)> | 15.84 | |
Completeness [%] | 100.0 | |
Redundancy | 6.9 | |
CC(1/2) | 0.999 | 0.865 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 0.2 M Potassium chloride, 0.05 M HEPES pH 7.5, 35% v/v Pentaerythritol propoxylate (5/4 PO/OH) |