7DY2
Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-03 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.803, 206.600, 168.383 |
Unit cell angles | 90.00, 94.69, 90.00 |
Refinement procedure
Resolution | 49.240 - 3.040 |
R-factor | 0.2662 |
Rwork | 0.263 |
R-free | 0.32560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gbl |
RMSD bond length | 0.002 |
RMSD bond angle | 1.245 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.150 |
High resolution limit [Å] | 3.038 | 3.038 |
Rmerge | 0.133 | 0.899 |
Number of reflections | 120308 | 12016 |
<I/σ(I)> | 15.6 | |
Completeness [%] | 99.9 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5 | 313 | Acetatic acid, Sodium formate |