7DY1
Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-19 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 131.103, 136.492, 190.772 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.280 - 2.200 |
| R-factor | 0.2352 |
| Rwork | 0.233 |
| R-free | 0.28420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o0m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.532 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 172130 | 8248 |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 99.7 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.989 | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5 | 313 | Acetatic acid, Sodium formate |
