7DY1

Crystal Structure of Cyanobacterial Circadian Clock Protein KaiC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-19
Detector	RAYONIX MX-225
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	131.103, 136.492, 190.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.280 - 2.200
R-factor	0.2352
Rwork	0.233
R-free	0.28420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o0m
RMSD bond length	0.008
RMSD bond angle	1.532
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	172130	8248
<I/σ(I)>	6.9
Completeness [%]	99.7
Redundancy	7.1
CC(1/2)	0.989	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5	313	Acetatic acid, Sodium formate