7DW7
Crystal Structure of N1051A mutant of Formylglycinamidine Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-10-25 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.587 |
| Spacegroup name | P 65 |
| Unit cell lengths | 146.626, 146.626, 141.022 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.990 - 1.800 |
| R-factor | 0.1345 |
| Rwork | 0.133 |
| R-free | 0.16250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t3t |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.281 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.023 | |
| Number of reflections | 158641 | 7938 |
| <I/σ(I)> | 34.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 8 | |
| CC(1/2) | 0.931 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2M Ammonium Sulphate |
