7DVV
Heme sensor protein PefR from Streptococcus agalactiae bound to operator DNA (28-mer)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 100.973, 100.973, 128.989 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.060 - 2.490 |
R-factor | 0.2189 |
Rwork | 0.217 |
R-free | 0.24630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7dvs 4lln |
RMSD bond length | 0.010 |
RMSD bond angle | 1.885 |
Data reduction software | XDS (Jun 1, 2017) |
Data scaling software | XSCALE (Jun 1, 2017) |
Phasing software | PHASER (2.7.17) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.060 | 47.060 | 2.650 |
High resolution limit [Å] | 2.490 | 7.400 | 2.490 |
Rmerge | 0.083 | 0.028 | 1.769 |
Rmeas | 0.089 | 0.030 | 1.911 |
Total number of observations | 357952 | ||
Number of reflections | 26423 | 1976 | 8222 |
<I/σ(I)> | 14.63 | 56.8 | 1 |
Completeness [%] | 99.9 | 99.4 | 99.4 |
Redundancy | 6.992 | 7.07 | 7.052 |
CC(1/2) | 0.999 | 0.999 | 0.562 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25%(w/v) PEGMME 2000, 0.2 M lithium sulfate, 0.1 M MES |