7DUJ

Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with mRNA and cognate transfer RNA anticodon stem-loop and sisomicin derivative N1,3''Bz bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9898
Spacegroup name	P 41 21 2
Unit cell lengths	400.000, 400.000, 173.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.801 - 3.750
R-factor	0.1893
Rwork	0.189
R-free	0.23490
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (DEV-3318)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.801	39.801	2.990
High resolution limit [Å]	2.910	13.030	2.910
Rmerge	1.212	0.071
Rmeas	1.221	0.071
Total number of observations	20503703
Number of reflections	292041	3538	16066
<I/σ(I)>	4.38	62.96
Completeness [%]	96.6	94.7	72.3
Redundancy	70.208	88.479	18.281
CC(1/2)	0.995	1.000	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	17% MPD