7DTS

Crystal structure of RNase L in complex with AC40357

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-01-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	59.010, 111.280, 264.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.355 - 2.630
R-factor	0.1957
Rwork	0.192
R-free	0.27030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m11
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.550	2.730
High resolution limit [Å]	2.630	2.630
Rmerge		1.297
Number of reflections	52595	5182
<I/σ(I)>	1.45
Completeness [%]	99.5
Redundancy	6.3
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% PEG4000, 100 mM Tris, 150 mM (NH4)2SO4 and 2 mM DTT buffer, pH 7.5