7DSA

Crystal structure of actin capping protein in complex with V-1 (space group P62)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	90
Detector technology	CCD
Collection date	2020-11-26
Detector	ADSC QUANTUM 270
Wavelength(s)	1.12
Spacegroup name	P 62
Unit cell lengths	160.293, 160.293, 51.999
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.270 - 2.800
R-factor	0.215
Rwork	0.213
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3aaa
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.270	46.270	2.970
High resolution limit [Å]	2.800	8.280	2.800
Rmerge	0.266	0.042	1.366
Rmeas	0.279	0.044	1.433
Total number of observations	212512
Number of reflections	19202	791	3061
<I/σ(I)>	11.71	45.9	2.1
Completeness [%]	100.0	99.4	100
Redundancy	11.067	10.024	10.97
CC(1/2)	0.993	1.000	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% (w/v) PEG 3350, 0.2M Magnesium Acetate