7DQ0
Crystal structure of actinomycin D-echinomycin-d(ACGTGCT/AGCTCGT) complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-19 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.00000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 30.188, 30.188, 137.608 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.033 | 0.016 | 0.230 |
Rmeas | 0.035 | 0.017 | 0.244 |
Rpim | 0.012 | 0.007 | 0.081 |
Number of reflections | 7257 | 553 | 445 |
<I/σ(I)> | 12.3 | ||
Completeness [%] | 98.8 | 96.3 | 98.5 |
Redundancy | 9.7 | 6.3 | 8.3 |
CC(1/2) | 0.999 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.5 mM Sodium Cacodylate, 1 mM Magnesium chloride, 1 mM Spermine tetrahydrochloride, 1.5 mM Zinc Chloride, 1% PEG 200 |