7DPI
Plasmodium falciparum cytoplasmic Phenylalanyl-tRNA synthetase in complex with BRD7929
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-17 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.648, 108.034, 140.532 |
| Unit cell angles | 90.00, 105.11, 90.00 |
Refinement procedure
| Resolution | 66.142 - 3.597 |
| R-factor | 0.2988 |
| Rwork | 0.294 |
| R-free | 0.33020 |
| Structure solution method | SAD |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15RC1_3423) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.142 | 66.150 | 3.660 |
| High resolution limit [Å] | 3.597 | 9.760 | 3.600 |
| Rmeas | 0.387 | 0.091 | 2.690 |
| Rpim | 0.146 | 0.035 | 1.023 |
| Total number of observations | 9914 | 9504 | |
| Number of reflections | 29013 | 1495 | 1383 |
| <I/σ(I)> | 3.1 | 6.8 | 0.8 |
| Completeness [%] | 99.1 | 98.5 | 95.8 |
| Redundancy | 6.9 | 6.6 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M sodium malonate, 12% w/v PEG 3350, 5% v/v Ethyl acetate |
