7DNG

DARPin 63_B7 in complex with linear V3-crown (MN) peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 64
Unit cell lengths	94.530, 94.530, 39.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.930 - 1.420
R-factor	0.1789
Rwork	0.178
R-free	0.20520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SVX chain A
RMSD bond length	0.004
RMSD bond angle	0.612
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.930	1.471
High resolution limit [Å]	1.420	1.420
Rmerge	0.105	6.440
Rmeas	0.110
Number of reflections	38156	3784
<I/σ(I)>	13.72
Completeness [%]	100.0	99.92
Redundancy	12.1	11.9
CC(1/2)	1.000	0.117

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	30% Jeffamine ED-2001, 0.1M HEPES pH 7.0