7DLK
Crystal Structure of veratryl alcohol bound Dye Decolorizing peroxidase from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-19 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.988, 102.108, 105.470 |
| Unit cell angles | 87.84, 76.45, 83.22 |
Refinement procedure
| Resolution | 45.680 - 2.100 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kmm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.708 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.680 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 170472 | 23937 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 90.6 | |
| Redundancy | 3.38 | |
| CC(1/2) | 0.990 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 26% MPD, 0.05 M HEPES |
