7DL9

Crystal structure of nucleoside transporter NupG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9791
Spacegroup name	P 1
Unit cell lengths	42.874, 68.715, 79.027
Unit cell angles	81.11, 74.27, 84.02

Refinement procedure

Resolution	25.566 - 3.000
R-factor	0.2534
Rwork	0.250
R-free	0.27980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	predicted model by the robetta website
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.540	29.540	3.180
High resolution limit [Å]	3.000	9.000	3.000
Rmerge	0.153	0.058	0.452
Total number of observations	59909	2077	9914
Number of reflections	16931	617	2727
<I/σ(I)>	6.8	14.1	2.8
Completeness [%]	98.8	95.7	98.5
Redundancy	3.5	3.4	3.6
CC(1/2)	0.986	0.995	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	0.1 M Citrate sodium (pH=5), 0.1 M NaCl 0.1 M MgCl2 , 30% PEG 600